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Human seminal plasma contains both free radicals and antioxidants which could influence sperm viability in long-term storage. The objective was to assess the contribution of the antioxidants present in human seminal plasma to the development of reactive oxygen species and lipid peroxidation during its storage at -20 degrees C. Thirty ejaculates were collected from fertile men and pooled for the preparation of seminal plasma. Antioxidants were precipitated with 70% methanol and both pellets and supernatants were analysed for lipid peroxidation, free radicals and antioxidants. During storage at -20 degrees C, seminal plasma undergoes lipid peroxidation and lipid peroxidation-derived products generate free radicals. The oxidative events were observed in both pellets and supernatants and were more pronounced in the supernatant. The seminal plasma contains both the lipid peroxidation products and antioxidants at the same time. The overall effect was the decrease of the antioxidants in the supernatant and in the pellets of seminal plasma, due to lipid peroxidation. The lipid peroxidation products such as acrolein and malondialdehyde are formed in the seminal plasma and may damage spermatozoa. Thiobarbituric acid reactive species were generated during storage at -20 degrees C and may affect spermatozoa. The availability of both the lipid peroxidation products and antioxidants could be one of the reasons for the decreased sperm viability during storage.Amsterdam – “State of the City” 2017
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