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Вместе недели. FaSuBacK.com по любим книгам, музыке.Beta-hydroxy-beta-methylglutaryl-CoA dehydrogenase
Beta-Hydroxy-beta-methylglutaryl-CoA dehydrogenase (, beta-hydroxy-beta-methylglutaryl coenzyme A dehydrogenase (isomerizing), beta-HMGCD) is an enzyme with systematic name beta-hydroxy-beta-methylglutaryl-CoA:acceptor oxidoreductase. This enzyme catalyses the following chemical reaction
beta-Hydroxy-beta-methylglutaryl-CoA + acceptor CoA + beta-hydroxy-beta-methylglutaryl-CoA-S-enol + 2 oxidized acceptor
This enzyme is isolated from the Gram-negative bacterium Escherichia coli.
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A microfluidic immunosensor was evaluated for detection of Salmonella Typhimurium, Listeria monocytogenes and Escherichia coli O157:H7. The system employs an antibody immobilized layer that is designed to capture the bacteria in a flow-through channel. The signal generated by the colorimetric reaction is detected by using a photodiode array as the detector. Several detection schemes were investigated. Two enzyme-linked sandwich assays were incorporated with the reaction sequence. A standard ELISA with conjugated antibodies was also tested for comparison. For ELISA and the enzyme-linked reactions the limit of detection (LOD) was determined for each pathogen. LOD for ELISA was 100 ng/mL for S. Typhimurium, 50 ng/mL for L. monocytogenes, and 25 ng/mL for E. coli O157:H7. The enzyme-linked reactions were compared to the ELISA by determination of the LOD. LOD for detection of S. Typhimurium was greater for the ELISA (5 ng/mL) than for the enzyme-linked reactions (1 ng/mL). For L. monocyt
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