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The objective of this study was to examine the induction of gill antibacterial defenses against a Gram-negative bacterium, Aeromonas hydrophila, in channel catfish after exposure to the antibacterial peptide, azurocidin. The cytotoxicity of azurocidin against Vibrio anguillarum and A. hydrophila was examined by incubating suspensions of cells in phosphate-buffered saline with various dilutions of azurocidin. The antibacterial activity of azurocidin was evaluated in a whole-body maceration assay and a cell-free extract assay. In a maceration assay, catfish exposed to azurocidin exhibited a concentration-dependent reduction in the mortality of A. hydrophila at 24 h, compared with catfish not exposed to azurocidin. A. hydrophila was resistant to azurocidin in the cell-free extract assay; however, when cell-free extracts of the azurocidin-treated catfish were examined in the extract assay, significant reductions in the growth of A. hydrophila in cell-free extracts were observed. These data suggest that azurocidin may possess antibacterial activity and/or may affect the induction of defense mechanisms in gill tissue of channel catfish. Although the mechanism(s) underlying the antibacterial activity of azurocidin are not fully elucidated, the present study provides the first evidence of protection against an important Gram-negative bacterial pathogen, A. hydrophila, in catfish gill tissue after exposure to the antibacterial peptide, azurocidin.1. Field of the Invention
This invention relates to an injection molding machine and particularly to an injection molding machine having a control apparatus for performing the shot count control of the control of the number of shots of the injection molding machine.
2. Prior Art
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