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In order to determine the effect of captopril (CTP), a potent inhibitor of angiotensin-converting enzyme, on differentiation of C2C12 myoblast, C2C12 myoblasts were cultured in media containing 0.5 x 10(-5) mol l(-1) CTP. CTP significantly inhibited the protein expression of myosin heavy chain and the synthesis of creatine kinase at concentrations greater than 10(-7) mol l(-1). On the other hand, CTP did not inhibit the proliferation of C2C12 myoblasts. These results suggest that CTP inhibits myogenic differentiation of C2C12 myoblasts and muscle cells, which may be related to inhibition of conversion of angiotensin II to angiotensin (1-7).1. Field of the Invention
The present invention relates to an improvement in the manufacturing process
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